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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

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Published on: August 8, 2016

Enzyme assays.

Jean-Louis Reymond1, Viviana S Fluxà, Noélie Maillard

  • 1Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, Berne, 3012, Switzerland. jean-louis.reymond@ioc.unibe.ch

Chemical Communications (Cambridge, England)
|December 17, 2008
PubMed
Summary
This summary is machine-generated.

Enzyme assays visualize enzyme activity for industrial enzyme discovery. This article focuses on fluorogenic and chromogenic substrates, sensors, and enzyme fingerprinting for white biotechnology applications.

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Enzymology

Background:

  • Enzyme assays are crucial analytical tools for understanding enzyme functions.
  • The development of novel enzyme assays aids in the discovery and optimization of industrial enzymes.
  • White biotechnology utilizes selective enzymes for sustainable and cost-effective production processes.

Purpose of the Study:

  • To highlight advancements in enzyme assay technologies.
  • To focus on specific areas including fluorogenic and chromogenic substrates, sensors, and enzyme fingerprinting.
  • To underscore the importance of these assays in industrial enzyme applications.

Main Methods:

  • Review of fluorogenic and chromogenic substrate-based enzyme assays.
  • Discussion of sensor technologies for enzyme activity detection.
  • Exploration of enzyme fingerprinting techniques for enzyme characterization.

Main Results:

  • Fluorogenic and chromogenic substrates offer sensitive detection of enzyme activities.
  • Enzyme sensors provide real-time monitoring capabilities.
  • Enzyme fingerprinting enables detailed characterization and differentiation of enzymes.

Conclusions:

  • Enzyme assays, particularly those utilizing advanced substrates and sensors, are vital for industrial enzyme development.
  • Fluorogenic/chromogenic substrates, sensors, and fingerprinting are key areas for innovation in enzyme assay technology.
  • These advancements support the growth of white biotechnology through efficient and sustainable enzyme applications.