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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

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A simple and sensitive colorimetric pH meter based on DNA conformational switch and gold nanoparticle aggregation.

Cuie Chen¹, Guangtao Song, Jinsong Ren

¹State Key Laboratory of Rare Earth Resources Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022.

Chemical Communications (Cambridge, England)

|December 17, 2008

Summary

A novel colorimetric pH meter utilizes DNA conformational changes and gold nanoparticle aggregation for rapid measurements. This nanotechnology achieves high accuracy across the physiological pH range.

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Area of Science:

Biotechnology
Nanotechnology
Analytical Chemistry

Background:

Accurate and rapid pH monitoring is crucial in various scientific fields.
Existing pH measurement methods can be complex or slow.

Purpose of the Study:

To develop a simple, rapid, and accurate colorimetric pH meter.
To leverage the properties of i-motif DNA and gold nanoparticles (AuNPs) for pH sensing.

Main Methods:

Development of a pH meter based on the conformational switch of i-motif DNA.
Utilizing non-crosslinking gold nanoparticle aggregation as a colorimetric indicator.
Testing the device's accuracy across the physiological pH range.

Main Results:

A functional colorimetric pH meter was successfully developed.
The device demonstrated rapid and simple operation.
Average accuracy was determined to be +/-0.04 pH unit within the physiological range.

Conclusions:

The developed i-motif DNA and AuNP-based sensor offers a promising new method for pH measurement.
This nanotechnology provides a simple and accurate alternative for real-time pH monitoring.