Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Complexometric Titration: Overview00:39

Complexometric Titration: Overview

Complexometric titration involves the formation of a complex by reacting a metal ion with one or more ligands. A visual indicator often detects the end point of a complexometric titration. It is added to the metal solution before the titration, forming a stable metal–indicator complex and imparting color to the solution. As the titration approaches the equivalence point, the excess of the added ligand displaces the indicator from the metal–indicator complex, releasing the free indicator. The...
Effects of EDTA on End-Point Detection Methods01:18

Effects of EDTA on End-Point Detection Methods

Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
Indicators02:39

Indicators

Certain organic substances change color in dilute solution when the hydronium ion concentration reaches a particular value. For example, phenolphthalein is a colorless substance in any aqueous solution with a hydronium ion concentration greater than 5.0 × 10−9 M (pH < 8.3). In more basic solutions where the hydronium ion concentration is less than 5.0 × 10−9 M (pH > 8.3), it is red or pink. Substances such as phenolphthalein, which can be used to determine the pH of a solution, are called...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Arsenite methyltransferase 3 is required for mitochondrial function and hepatic lipid metabolism.

The FEBS journal·2026
Same author

Targeted and Nontargeted Detection and Quantitation of Arsenolipids in a Tuna Fish Reference Material (BCR-627) Using Reversed-Phase HPLC with High-Resolution Electrospray Mass Spectrometry and Inductively Coupled Plasma Mass Spectrometry.

Analytical chemistry·2026
Same author

From Nature to Synthesis and Vice Versa: Costic Acid Analogs with Acaricidal Activity Against the Bee Parasite <i>Varroa destructor</i>.

Plants (Basel, Switzerland)·2026
Same author

Nickel-induced multimetal uptake in two microalgal species (Chlorella sorokiniana and Chlamydomonas reinhardtii) and its effect on growth and lipid unsaturation.

Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)·2024
Same author

Deciphering the associations of selenium distribution in serum GPx-3 and selenoprotein P with cardiovascular risk factors in a healthy population with moderate levels of selenium: The ATTICA study.

Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)·2024
Same author

Continuous oxygen monitoring to enhance ex-vivo organ machine perfusion and reconstructive surgery.

Biosensors & bioelectronics·2024

Related Experiment Video

Updated: Jun 27, 2026

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels
10:07

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels

Published on: January 27, 2013

Coumarin-based ratiometric fluorescent indicators with high specificity for lead ions.

Emmanuel Roussakis1, Spiros A Pergantis, Haralambos E Katerinopoulos

  • 1Department of Chemistry, University of Crete, Voutes Campus, Crete, 71003 Heraklion, Greece.

Chemical Communications (Cambridge, England)
|December 17, 2008
PubMed
Summary

This study reports the total synthesis and spectral properties of novel fluorescent indicators designed for detecting lead(II) ions. These new compounds offer enhanced capabilities for lead(II) detection in various applications.

More Related Videos

Iridium(III) Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II
12:52

Iridium(III) Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

Published on: July 7, 2015

Related Experiment Videos

Last Updated: Jun 27, 2026

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels
10:07

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels

Published on: January 27, 2013

Iridium(III) Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II
12:52

Iridium(III) Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

Published on: July 7, 2015

Area of Science:

  • Chemical synthesis
  • Analytical chemistry
  • Spectroscopy

Background:

  • Lead(II) ions are toxic environmental pollutants.
  • Accurate detection of lead(II) is crucial for environmental monitoring and biological studies.
  • Development of sensitive and selective fluorescent indicators is an active area of research.

Purpose of the Study:

  • To report the total synthesis of novel fluorescent indicators for lead(II) ions.
  • To characterize the spectral properties of these newly synthesized compounds.
  • To evaluate their potential as tools for lead(II) detection.

Main Methods:

  • Total chemical synthesis of fluorescent molecules.
  • Spectroscopic analysis including UV-Vis absorption and fluorescence emission.
  • Characterization of photophysical properties.

Main Results:

  • Successful synthesis of new fluorescent lead(II) indicators.
  • Detailed spectral data and photophysical properties were obtained.
  • The indicators exhibit fluorescence changes upon binding with lead(II) ions.

Conclusions:

  • The synthesized compounds are effective fluorescent indicators for lead(II).
  • These indicators possess favorable spectral properties for detection applications.
  • The developed indicators show promise for environmental and biological sensing of lead(II).