Related Experiment Video
Updated: Jun 27, 2026

Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry
Published on: April 9, 2021
Using acridinium ester as the sonochemiluminescent probe for labeling of protein
Yongquan Lai1, Yuanyuan Qi, Jian Wang
1Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety (Fuzhou University), and Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China.
Abstract:
A new sonogenerated chemiluminescence (SCL) system has been developed to investigate the SCL behavior of acidinium ester (AE). The optimum parameters for this SCL system have also been investigated in detail. Under the optimum conditions, a typical SCL signal could be obtained in a mixture of DMF and alkaline water solutions with volume ratio of 3:2. According to electrospray ionization mass spectrometry and sonochemiluminescence emission spectra, SCL emission could be ascribed to the formation of N-methylacridone with a different mechanism from that in both the chemiluminescence and electrochemiluminescence reaction of acridinium ester. The acridinium ester has been used as a SCL probe for labeling of anti-human insulin guinea pig polyclonal antibody, and it was found that the concentration of AE-conjugated antibody was linear with the SCL intensity in the range of 0.15-0.75 ppb with a linear correlation coefficient of 0.9980.
More Related Videos
Related Concept Videos
Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Immunogold Electron Microscopy
Protein Dynamics in Living Cells
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

