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Updated: Jun 27, 2026

Harmonic Nanoparticles for Regenerative Research
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Rapid cell mapping using nanoparticles and SERRS.

Robert J Stokes1, Fiona McKenzie, Emma McFarlane

  • 1Centre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow, UKG1 1XL.

The Analyst
|December 17, 2008
PubMed
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Gold and silver nanoparticles enhance cell mapping. This novel surface-enhanced resonance Raman scattering (SERRS) method identifies nanoparticle aggregates in immune cells, offering superior sensitivity and speed for biological imaging.

Area of Science:

  • Cellular imaging and analysis
  • Nanoparticle-based diagnostics
  • Spectroscopic techniques in biology

Background:

  • Macrophages are crucial immune cells involved in various biological processes.
  • Current cell staining and imaging methods can have limitations in sensitivity and specificity.
  • Nanoparticles offer unique optical properties for enhanced detection.

Purpose of the Study:

  • To investigate the use of gold and silver nanoparticles with surface-enhanced resonance Raman scattering (SERRS) for enhanced cell mapping.
  • To evaluate the selectivity and sensitivity of SERRS mapping for identifying nanoparticle aggregates within cells.
  • To demonstrate the potential of combining nanoparticles and cell stains for improved cellular analysis.

Main Methods:

  • Bone marrow-derived immune cells (macrophages) were treated with gold and silver nanoparticles.

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  • Cells underwent fixation and dye staining.
  • Multiple wavelength line scanning SERRS mapping was employed for analysis.

    • Main Results:

    • The SERRS mapping method demonstrated high selectivity and sensitivity.
    • Nanoparticle aggregates were successfully identified within secondary lysosomes of macrophages.
    • Quantification of fluorescence, Raman, and SERRS signals at four excitation wavelengths was achieved.

    Conclusions:

    • The developed SERRS method enables rapid and precise identification of nanoparticle aggregates in immune cells.
    • Combining nanoparticles with routine cell stains offers superior cell mapping capabilities.
    • This approach shows promise for advanced biological imaging at longer, biologically compatible wavelengths.