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Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation
Published on: November 10, 2017
Cyclin A1 regulates WT1 expression in acute myeloid leukemia cells
Utz Krug1, Amber Yasmeen, Carmela Beger
1Department of Medicine, Hematology and Oncology, University Hospital of Münster, 48129 Münster, Germany.
International Journal of Oncology
|December 17, 2008
Summary
Cyclin A1, a cell cycle protein, represses WT1 expression in hematopoietic cells. This suppression of WT1 by cyclin A1 may contribute to the growth and survival of leukemic cells.
Area of Science:
- Hematology
- Molecular Biology
- Cancer Research
Background:
- Cyclin A1 is a cell cycle protein found in testes, brain, and hematopoietic progenitor cells.
- Overexpression of Cyclin A1 is observed in myeloid leukemic cell lines and blasts, and can induce acute myeloid leukemia in mice.
Purpose of the Study:
- To identify target genes of Cyclin A1 in hematopoietic cells.
- To investigate the relationship between Cyclin A1 and WT1 expression in leukemia.
Main Methods:
- Microarray analysis of U937 myeloid cells with and without Cyclin A1 overexpression.
- Confirmation of WT1 repression at mRNA and protein levels.
- Analysis of WT1 isoforms and functional assays in U937 cells.
Main Results:
- Cyclin A1 overexpression led to differential expression of 35 genes, including repression of WT1.
- WT1 expression was higher in Cyclin A1-deficient mice.
- WT1 isoform ratios were altered by Cyclin A1 overexpression, affecting cell cycle arrest.
Conclusions:
- WT1 is identified as a repressed target gene of Cyclin A1.
- Suppression of WT1 by Cyclin A1 may promote leukemic cell growth and inhibit apoptosis.
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