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Profiling of transcripts and proteins modulated by K-ras oncogene in the lung tissues of K-ras transgenic mice by

Sojung Lee1, Jungwoo Kang, Minchul Cho

  • 1Department of Bioscience and Biotechnology, Konkuk University, Gwangjin-gu, Seoul 143-701, Korea.

International Journal of Oncology
|December 17, 2008
PubMed

Insights

The K-ras oncogene promotes cancer by up-regulating genes linked to tumor growth and inflammation while down-regulating tumor suppressor genes. This research identifies potential biomarkers for cancer diagnosis and prevention.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • The mutated K-ras gene is implicated in approximately 30% of human cancers.
  • Understanding K-ras oncogene-induced molecular changes is crucial for cancer research.

Purpose of the Study:

  • To identify K-ras oncogene-induced modulators in lung tissues of K-ras transgenic mice.
  • To explore potential biomarkers for cancer diagnosis and prevention.

Main Methods:

  • Microarray analysis to assess gene expression.
  • Proteomics (LC/ESI-MS/MS) to identify protein level changes.
  • Utilized K-ras transgenic mouse models for lung tissue analysis.

Main Results:

  • K-ras oncogene up-regulated cancer/inflammation-related genes (e.g., Wnt, chemokine/cytokine signaling) and down-regulated tumor suppressor genes (e.g., p53, TGF-beta pathways).
  • Proteomics revealed increased metabolism/catabolism, translation/transcription, signal transduction, and phosphorylation proteins.
  • Identified specific up-regulated proteins (e.g., ATP synthase, Ras family) and down-regulated proteins (e.g., integrin alpha1, Rain).

Conclusions:

  • K-ras oncogene drives tumor growth by altering gene and protein expression, favoring cancer development and suppressing tumor suppression mechanisms.
  • Identified potential biomarkers (e.g., Cdc37, Glycam 1, IL-33, Ccl6, epiregulin) for cancer diagnosis, prognosis, and therapeutic targets.

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