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Updated: Jun 27, 2026

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
Published on: June 11, 2015
Phage proteomics: applications of mass spectrometry
Rob Lavigne1, Pieter-Jan Ceyssens, J Robben
1Department of Biosystems, Division of Gene Technology, Katholieke Universiteit Leuven, Leuven, Belgium.
Mass spectrometry (MS) identifies phage structural proteins. Electron spray ionization tandem mass spectrometry (ESI-MS/MS) systematically analyzes the phage proteome, confirming genome predictions.
Area of Science:
- Proteomics
- Mass Spectrometry
- Molecular Biology
Background:
- Protein identification relies on mass spectrometry (MS) and sequence databases.
- Phage structural proteins are crucial for viral structure and function.
- Genome predictions require experimental validation.
Purpose of the Study:
- To provide an overview of MS techniques for phage structural protein identification.
- To detail an electron spray peptide ionization tandem mass spectrometry (ESI-MS/MS) approach.
- To systematically identify the phage structural proteome.
Main Methods:
- Utilizing mass spectrometry (MS) for protein identification.
- Employing electron spray peptide ionization tandem mass spectrometry (ESI-MS/MS).
- Analyzing phage structural proteome comprehensively.
Main Results:
- Sensitive and accurate identification of phage structural proteins.
- Systematic experimental examination of the phage proteome.
- Confirmation of genome-based gene predictions for phage proteins.
Conclusions:
- ESI-MS/MS is effective for comprehensive phage proteome analysis.
- Experimental validation confirms in silico predictions.
- MS techniques are vital for understanding phage biology.
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