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Published on: September 1, 2017
Determination of seven arsenic compounds in urine by HPLC-ICP-DRC-MS: a CDC population biomonitoring method
Carl P Verdon1, Kathleen L Caldwell, Mark R Fresquez
1Inorganic and Radiation Analytical Toxicology Branch, Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Highway, N.E., Mail Stop F-50, Atlanta, GA 30341, USA. cverdon@cdc.gov
Abstract:
A robust analytical method has been developed and validated by use of high-performance liquid chromatography inductively coupled plasma mass spectrometry with Dynamic Reaction Cell (DRC) technology that separates seven arsenic (As) species in human urine: arsenobetaine (AB), arsenocholine, trimethylarsine oxide (TMAO), arsenate (As(V)), arsenite (As(III)), monomethylarsonate, and dimethylarsinate. A polymeric anion-exchange (Hamilton PRP X-100) column was used for separation of the species that were detected at m/z 75 by ICP-DRC-MS (PerkinElmer SCIEX ELAN DRCII) using 10% hydrogen-90% argon as the DRC gas. The internal standard (As) is added postcolumn via an external injector with a sample loop. All analyte peaks were baseline-separated except AB and TMAO. Analytical method limits of detection for the various species ranged from 0.4 to 1.7 microg L(-1) as elemental As. As(III) conversion to As(V) was avoided by adjusting the urine sample to
