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Updated: Jun 27, 2026
Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
Published on: February 7, 2018
Correlative transmission microscopy: cytochemical localization and immunocytochemical localization in studies of
1Microscopy and Imaging Center, Texas A&M University, College Station, Texas.
Abstract:
Microscopy studies of oxidative stress have often consisted of only immunocytochemical localization or only cytochemical localization studies. However, correlative studies on the same sections provide more useful data in interpreting oxidative and nitrosative stress. Cellular sites of superoxide and hydrogen peroxide production can be identified using cerium-based cytochemical localization of NADH oxidase enzymatic activity, while areas of nitrosative stress can be identified by immunocytochemical localization of nitrotyrosine in the same sections of tissue.
