Related Experiment Video
Updated: Jun 27, 2026
Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
Detection of specifically oxidized apolipoproteins in oxidized HDL
Xiao Suo Wang1, Roland Stocker
1Centre for Vascular Research, Bosch Institute and Discipline of Pathology, The University of Sydney, Sydney, Australia.
Insights
Oxidized high-density lipoprotein (HDL) contributes to atherosclerosis. This study introduces a new HPLC method to detect oxidized forms of apoA-I and apoA-II in HDL, aiding early detection of HDL dysfunction.
Area of Science:
- Biochemistry
- Cardiovascular Research
- Analytical Chemistry
Background:
- Atherosclerosis is linked to dysfunctional high-density lipoprotein (HDL).
- Oxidation is a key process leading to HDL dysfunction.
- Lipid hydroperoxides (LOOHs) are primary products of lipoprotein oxidation.
Purpose of the Study:
- To develop a method for detecting oxidized forms of apolipoprotein (apo) A-I and apoA-II in HDL.
- To assess early stages of HDL oxidation in biological samples.
Main Methods:
- HPLC-based detection of oxidized HDL.
- Identification of oxidized methionine residues (MetO) in apoA-I and apoA-II.
Main Results:
- A novel HPLC method was established to detect specific oxidized forms of apoA-I and apoA-II.
- The method identifies MetO modifications on apoA-I and apoA-II, indicating HDL oxidation.
Conclusions:
- The described HPLC method can identify early-stage HDL oxidation.
- This technique may be valuable for studying HDL dysfunction in atherosclerosis.
Abstract:
Atherosclerosis is associated with dysfunctional HDL, and oxidation of HDL is thought to give rise to HDL becoming dysfunctional. Lipoprotein oxidation represents a complex series of processes that can be assessed by various methods. In general, oxidation mediated by 1-electron or radical oxidants gives rise to lipid hydroperoxides (LOOHs) as the primary product. These LOOHs may then undergo further reactions giving rise to secondary lipid oxidation products and/or oxidation of lipoprotein-associated proteins. Thus, LOOHs specifically oxidize Met residues of apolipoprotein (apo) A-I and A-II (the major proteins of HDL) to MetO. Here we describe an HPLC-based method to detect oxidized HDL containing specifically oxidized forms of apoA-I and apoA-II. This method may be useful to assess the early stages of HDL oxidation in biological samples.
