Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Factors Influencing Functional Recovery in People After Chronic Critical Illness During Early Neurological Rehabilitation.

Journal of multidisciplinary healthcare·2026

Same author

The plant ARF GTPase family: current perspectives in membrane trafficking, cellular signalling and new paradigms.

Plant & cell physiology·2026

Same author

Health economic evaluation of an interdisciplinary care pathway for older patients with vertigo, dizziness and balance disorders in primary care (MobilE-PHY2) - a cluster-randomised trial.

Cost effectiveness and resource allocation : C/E·2026

Same author

Multilayered regulation of Fe excess stress and the role of ascorbate in rice.

Protoplasma·2026

Same author

Japanese soil-borne wheat mosaic virus CP-RT protein associates with the endoplasmic reticulum network and mobile particles at the ER-Golgi interface during infection.

Protoplasma·2026

Same author

Root cell wall plasticity in iron homeostasis: an overlooked frontier in plant nutrition.

The New phytologist·2025

Search research articles

Related Experiment Video

Updated: Jun 27, 2026

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

Accurate Real-time Reverse Transcription Quantitative PCR.

Marco Klatte¹, Petra Bauer

¹Department of Biosciences, Botany, Saarland University, Saarbruecken, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|December 17, 2008

Summary

Real-time quantitative PCR offers highly sensitive mRNA quantification. This protocol simplifies establishing accurate real-time RT-qPCR for reliable gene expression analysis.

Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

Real-time quantitative PCR (qPCR) is the preferred method for accurate mRNA quantification.
Sensitivity has increased to single-molecule detection limits.
Higher sensitivity necessitates rigorous experimental design.

Purpose of the Study:

To present an approved protocol for establishing mRNA quantification using real-time RT-qPCR.
To simplify the process of implementing sensitive gene expression analysis.

Main Methods:

Detailed protocol for real-time RT-qPCR implementation.
Focus on experimental design considerations for high sensitivity.

Main Results:

A straightforward and approved method for real-time RT-qPCR established.

More Related Videos

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Published on: March 31, 2021

Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR

Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR

Published on: December 6, 2014

Related Experiment Videos

Last Updated: Jun 27, 2026

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Published on: March 31, 2021

Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR

Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR

Published on: December 6, 2014

Enables reliable mRNA quantification at high sensitivity.

Conclusions:

The described protocol facilitates the adoption of sensitive real-time RT-qPCR.
A simplified approach for accurate gene expression analysis is provided.