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JC viral DNA chip allows geographical localization of unidentified cadavers for rapid identification
Hiroshi Ikegaya1, Hirotaro Iwase, Pekka J Saukko
1National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan. ikegaya-tky@umin.ac.jp
Abstract:
To narrow down the geographical origins of unidentified cadavers, a rapid and simple method to detect JC virus (JCV) genotypes using a DNA chip was developed. Fifty-four probes on a 3mm silicon DNA chip were designed to distinguish 12 JCV genotypes. The detection limit of the DNA chip was 0.001 pg/microL of template JCV DNA concentration, showing a sensitivity equal to that of the conventional method. The analysis requires only a few hours to yield results. This new DNA chip method helps to narrow the search area for human identification much more quickly and easily than use of the conventional method.
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