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Updated: Jun 27, 2026

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
Haplotype diversity in human mitochondrial DNA hypervariable regions I-III in the city of Caracas (Venezuela)
N Lander1, M G Rojas, M A Chiurillo
1Centro de Biotecnología, Fundación Instituto de Estudios Avanzados (IDEA), Caracas, Venezuela.
Abstract:
In order to expand the database of variable DNA for forensic identification purposes in Venezuela, we analyzed the sequence polymorphisms of mitochondrial DNA (mtDNA) hypervariable regions (HVR) I-III from 100 unrelated individuals from the city of Caracas, using PCR amplification and fluorescent-based capillary electrophoresis sequencing method. Dominant haplogroups corresponded to Native Americans followed by African ones. The inclusion of HVR III although useful for sub-haplogroup assignation, added little to the discrimination capacity of our database.
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