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Updated: Jun 27, 2026

Continuously-stirred Anaerobic Digester to Convert Organic Wastes into Biogas: System Setup and Basic Operation
Published on: July 13, 2012
Effect of post-digestion temperature on serial CSTR biogas reactor performance.
Kanokwan Boe1, Dimitar Karakashev, Eric Trably
1Department of Environmental Engineering, Technical University of Denmark, Building 113, DK-2800 Kgs. Lyngby, Denmark.
Optimizing post-digestion temperature in continuous-flow stirred tank reactors (CSTRs) significantly boosts biogas production. Maintaining higher temperatures, particularly 55°C, enhances system stability and microbial activity for greater energy recovery.
Area of Science:
- Environmental Engineering
- Biotechnology
- Anaerobic Digestion
Background:
- Continuous-flow stirred tank reactors (CSTRs) are widely used for waste treatment and biogas generation.
- Post-digestion stages can further enhance biogas yield and process stability.
- Temperature is a critical factor influencing microbial activity in anaerobic digestion.
Purpose of the Study:
- To investigate the impact of post-digestion temperature on the performance of a lab-scale CSTR system.
- To compare the effects of 55°C, 37°C, and 15°C on biogas production, stability, microbial community, and methanogenic activity.
Main Methods:
- A serial CSTR system with a main reactor (55°C, 15 days HRT) followed by post-digesters (5.3 days HRT) was employed.
- Three different post-digestion temperatures (55°C, 37°C, 15°C) were tested.
- Biogas production, volatile fatty acid (VFA) concentrations, microbial community, and specific methanogenic activity were analyzed.
Main Results:
- Post-digestion at 55°C, 37°C, and 15°C resulted in additional biogas production of 11.7%, 8.4%, and 1.2%, respectively.
- The 55°C post-digester exhibited the highest biogas yield and superior stability, indicated by low VFA levels.
- High acidogen and methanogen activity was observed at 55°C and 37°C, while activity was minimal at 15°C.
Conclusions:
- Post-digestion temperature significantly influences biogas production and process stability in CSTR systems.
- Operating post-digesters at mesophilic temperatures (55°C and 37°C) maximizes biogas yield and microbial activity.
- Lower temperatures (15°C) drastically reduce methanogenic activity and overall system performance.
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