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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jun 27, 2026

Methylated DNA Immunoprecipitation
21:24

Methylated DNA Immunoprecipitation

Published on: January 2, 2009

Detection method for methylation density on microarray using methyl-CpG-binding domain protein.

Junfeng Luo1, Wenli Zheng, Yan Wang

  • 1State Key Laboratory of Bioelectronics, Department of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.

Analytical Biochemistry
|December 17, 2008
PubMed
Summary
This summary is machine-generated.

A novel DNA microarray method quantifies DNA methylation density in target CpG islands. This technique accurately assesses methylation levels in genes like CDK2A and CDK2B, crucial for cancer research.

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Last Updated: Jun 27, 2026

Methylated DNA Immunoprecipitation
21:24

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Published on: January 2, 2009

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08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

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Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
13:21

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Published on: June 16, 2017

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Genomics

Background:

  • DNA methylation is a critical epigenetic modification influencing gene expression.
  • Aberrant DNA methylation patterns are hallmarks of various diseases, particularly cancer.
  • Accurate quantification of methylation density is essential for understanding disease mechanisms.

Purpose of the Study:

  • To establish a novel method for determining the methylation density of target CpG islands.
  • To enable quantitative analysis of regional methylation in specific genes.
  • To provide a scalable approach for analyzing methylation in clinical samples.

Main Methods:

  • Development of a DNA microarray technique using bisulfite-converted DNA.
  • Treatment of PCR products with SssI methyltransferase and labeling with TAMRA fluorescence.
  • Utilizing a methyl-CpG-binding protein labeled with Cy5 fluorescence for signal detection.
  • Establishing a standard curve for quantitative assessment of methylation density.

Main Results:

  • Successfully determined the methylation density of six CpG islands in CDK2A and CDK2B genes.
  • Analyzed samples from seven cancer cell lines and two normal blood samples.
  • Validated the method's accuracy through comparison with bisulfite sequencing.
  • Demonstrated the method's capability for quantitative analysis of regional methylation.

Conclusions:

  • The established DNA microarray method provides a reliable tool for quantitative analysis of DNA methylation density.
  • This technique is applicable to a set of given genes and can be scaled for large numbers of clinical samples.
  • The method offers valuable insights into epigenetic alterations in cancer and other diseases.