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Updated: Jun 27, 2026

Single-Molecule Imaging of Nuclear Transport
Published on: June 9, 2010
Nuclear import of Pin1 is mediated by a novel sequence in the PPIase domain
1Institute of Molecular and Cell Biology, A *STAR (Agency for Science, Technology and Research), 61 Biopolis Drive, Biopolis, Singapore 138673, Republic of Singapore.
Abstract:
Pin1 actively regulates diverse biological/pathological processes, but little is known about the regulatory mechanisms of its cellular localization. In this study, we report that the endogenous Pin1 is distributed in both nucleus and cytoplasm. We found that point mutations of several basic amino acids in the PPIase domain of Pin1 significantly compromise its nuclear localization. Such inhibition is independent of Pin1 enzymatic activity, and is mainly due to the defects in the nuclear import. A novel sequence harboring these residues was identified as a putative nuclear localization signal (NLS) of Pin1. Importin alpha5 of the nuclear import machinery was found to interact with Pin1.
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