Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 27, 2026

Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test

Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test

Published on: July 14, 2023

Multiplexed Elispot assay.

William D Harriman¹, Ellen J Collarini, Remy G Cromer

¹Trellis Bioscience, 2-B Corporate Dr., South San Francisco, CA 94080, United States.

Journal of Immunological Methods

|December 17, 2008

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Effectiveness of a Bobath Concept-Based Nursing Model on Neurological Function and Independence in Daily Living After Arteriovenous Malformation Resection: A Retrospective Study.

British journal of hospital medicine (London, England : 2005)·2026

Same author

Finite element-guided optimization of personalized vacuum bell devices for pectus excavatum: an exploratory retrospective clinical study.

Pediatric surgery international·2026

Same author

Remodelling of body composition surrounding the diagnosis of type 2 diabetes in three longitudinal cohorts.

Diabetologia·2026

Same author

Extracellular Vesicles for Therapeutic Applications: A Translational Framework Integrating Sources, Administration Routes, Indications, Quality Control, and Regulatory Systems.

International journal of nanomedicine·2026

Same author

Dapagliflozin Improves Mitochondrial Function to Reduce the Risk of Atrial Fibrillation in a High-Glucose Environment by Activating the AMPK/PGC-1α/SIRT3 Signalling Pathway.

Clinical and experimental pharmacology & physiology·2026

Same author

Ultra-Low Pt Loading Bimetallic PtNi Catalyst on Nano-LTL Zeolite for the Selective Hydrogenation of Halonitrobenzenes.

Molecules (Basel, Switzerland)·2026

This study introduces novel 280 nm combinatorially labeled particles for ultrasensitive ELISA-style assays. This technique enables multiparametric profiling of cytokine footprints and concurrent identification of secreting T lymphocytes.

Area of Science:

Biotechnology
Immunology
Microscopy

Background:

Micron-scale latex beads are biocompatible reagents used in various biological assays.
Previous applications, such as flow cytometry, primarily utilized larger beads (approx. 5 micrometers).
Combinatorially colored labels can be created by incorporating two fluorescent dyes into latex beads.

Purpose of the Study:

To demonstrate the utility of 280 nm combinatorially labeled particles in novel ELISA-style assays.
To develop a high-throughput method for profiling cellular secretions.
To enable simultaneous identification of cell types and secreted analytes.

Main Methods:

Development of ELISA-style assays using 280 nm combinatorially labeled particles in 200 micrometer virtual wells.

More Related Videos

Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens

Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens

Published on: November 23, 2010

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

Related Experiment Videos

Last Updated: Jun 27, 2026

Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test

Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test

Published on: July 14, 2023

Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens

Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens

Published on: November 23, 2010

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

Utilizing digital microscopy for readout of the assay.

Application in a multiparametric Elispot assay for profiling peripheral blood mononuclear cells (PBMCs).

Concurrent surface staining with cell type-specific antibodies conjugated to the same beads.

Main Results:

Successful implementation of ELISA-style assays with 280 nm beads.
Profiling of secreted cytokine footprints from PBMCs.
Identification of previously unrecognized classes of T lymphocytes.
Demonstration of concurrent cell type identification and cytokine profiling.

Conclusions:

280 nm combinatorially labeled particles are effective for ultrasensitive ELISA-style assays.
This technique offers a powerful tool for multiparametric analysis of cellular secretions.
The method facilitates deeper insights into immune cell function and heterogeneity.