Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However, invadopodia can...
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tumour acidosis remodels the glycocalyx to control lipid scavenging and ferroptosis.

Nature cell biology·2026
Same author

Mitoxantrone alters CD24/Siglec-10 expression in malignant brain tumor models.

Scientific reports·2026
Same author

Extracellular vesicles as liquid biopsy tools for personalized therapeutic goals in glioblastoma.

Brain : a journal of neurology·2026
Same author

Noninvasive MGMT-promotor methylation prediction in high grade gliomas using conventional MRI and deep learning-based segmentations.

Frontiers in neuroscience·2026
Same author

Cellular and biophysical barriers to lipid nanoparticle mediated delivery of RNA to the cytosol.

Nature communications·2025
Same author

Availability and use of PET in patients with brain tumours - a European Organisation for Research and Treatment of Cancer - Brain Tumour Group (EORTC-BTG) survey.

European journal of nuclear medicine and molecular imaging·2025

Related Experiment Video

Updated: Jun 27, 2026

Initial Evaluation of Antibody-conjugates Modified with Viral-derived Peptides for Increasing Cellular Accumulation and Improving Tumor Targeting
11:58

Initial Evaluation of Antibody-conjugates Modified with Viral-derived Peptides for Increasing Cellular Accumulation and Improving Tumor Targeting

Published on: March 8, 2018

Characterizing peptide-mediated DNA internalization in human cancer cells.

Anders Wittrup1, Mattias Belting

  • 1Department of Clinical Sciences, Section of Oncology, Lund University, Sweden.

Methods in Molecular Biology (Clifton, N.J.)
|December 17, 2008
PubMed
Summary

Cell penetrating peptides (CPPs) primarily enter cells via endocytosis, not direct membrane crossing. This study details methods to characterize CPP uptake mechanisms and intracellular DNA delivery pathways.

More Related Videos

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
10:26

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions

Published on: December 20, 2017

Related Experiment Videos

Last Updated: Jun 27, 2026

Initial Evaluation of Antibody-conjugates Modified with Viral-derived Peptides for Increasing Cellular Accumulation and Improving Tumor Targeting
11:58

Initial Evaluation of Antibody-conjugates Modified with Viral-derived Peptides for Increasing Cellular Accumulation and Improving Tumor Targeting

Published on: March 8, 2018

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
10:26

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions

Published on: December 20, 2017

Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Delivery Systems

Background:

  • Cell penetrating peptides (CPPs) facilitate intracellular delivery of macromolecular cargos.
  • Evidence suggests CPPs predominantly enter cells via endocytosis, challenging the initial direct membrane penetration hypothesis.
  • The specific endocytic pathway for CPPs remains poorly defined and context-dependent.

Purpose of the Study:

  • To provide detailed protocols for characterizing the endocytosis mechanism of CPP-mediated DNA delivery.
  • To offer methods for quantitative and qualitative assessment of CPP uptake.
  • To investigate the intracellular fate of delivered DNA and the role of dynamin in the uptake process.

Main Methods:

  • Quantitative uptake analysis using fluorescence-assisted cell sorting (FACS).
  • Qualitative uptake assessment via confocal microscopy.
  • Intracellular fate determination through co-localization studies with endosomal markers (transferrin, dextran, caveolin-1).
  • Assessment of dynamin dependence using a dominant-negative dynamin-2 construct.

Main Results:

  • Established protocols for characterizing CPP uptake mechanisms.
  • Demonstrated co-localization of internalized DNA with specific endosomal markers.
  • Provided a method to evaluate the role of dynamin in CPP-mediated DNA delivery.

Conclusions:

  • The provided protocols enable robust characterization of CPP uptake pathways.
  • Understanding CPP endocytosis is crucial for optimizing intracellular delivery strategies.
  • Dynamin plays a significant role in the uptake of DNA-peptide complexes via CPPs.