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Effect of MT1-MMP deficiency and overexpression in corneal keratocytes on vascular endothelial cell migration and

Dimitri T Azar1, Fabio H Casanova, Tatsuya Mimura

  • 1Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, Illinois 60612, USA. dazar@uic.edu

Current Eye Research
|December 17, 2008
PubMed
Abstract

Insights

Keratocyte-derived MT1-MMP inhibits calf pulmonary artery endothelial cell (CPAE) proliferation and migration, suggesting anti-angiogenic properties. This research impacts understanding of blood vessel formation and related conditions.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Angiogenesis Research

Background:

  • Matrix metalloproteinase 1 (MT1-MMP) plays a role in extracellular matrix remodeling.
  • Endothelial cell proliferation and migration are crucial for angiogenesis.
  • Keratocytes are connective tissue cells that can influence their microenvironment.

Purpose of the Study:

  • To investigate the impact of keratocyte-derived MT1-MMP on the proliferation and migration of calf pulmonary artery endothelial cells (CPAE).

Main Methods:

  • Generated keratocyte cell lines from MT1-MMP knockout (KO) and wild-type (WT) mice.
  • Transfected WT keratocytes with WT or mutant MT1-MMP DNA.
  • Assayed the effect of conditioned media from these keratocytes on CPAE cell proliferation and migration.

Main Results:

  • Conditioned media from KO keratocytes significantly increased CPAE cell proliferation (190.5%).
  • WT keratocyte conditioned media showed higher CPAE proliferation (155.4%) compared to media from MT1-MMP-transfected keratocytes (119.7%).
  • Migration assays corroborated the observed effects on proliferation.

Conclusions:

  • Keratocyte-derived MT1-MMP exhibits anti-angiogenic effects on CPAE cells.
  • MT1-MMP expression in keratocytes influences endothelial cell behavior.
  • These findings contribute to understanding MT1-MMP's role in vascular biology.

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