Jove
Visualize
Contact Us

Related Experiment Video

Updated: Jun 27, 2026

Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching
07:32

Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching

Published on: June 30, 2010

Embryonic lung growth is normal in a cftr-knockout mouse model.

Helen L Wallace1, Marilyn G Connell, Paul D Losty

  • 1Department of Physiology, University of Liverpool, Liverpool, United Kingdom. hwallace@liv.ac.uk

Experimental Lung Research
|December 17, 2008
PubMed
Summary

Embryonic lung development and airway function are not affected by the absence of the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel. These findings indicate that CFTR is not essential for normal lung growth or airway peristalsis during embryonic development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Performance Evaluation of a Novel Cystic Fibrosis Caregiver Burden Measure Demonstrates Significant Challenges for Parents of Children With CF During the Early Years: The Irish Comparative Outcomes Study (ICOS).

Pediatric pulmonology·2026
Same author

CFTR modulator monotherapy for people with cystic fibrosis with class II CFTR gene variants (most commonly F508del).

The Cochrane database of systematic reviews·2026
Same author

Image defined risk factor(s) and outcomes for abdominal neuroblastoma: a surgical perspective from a Thailand National Cancer Centre.

Pediatric surgery international·2026
Same author

Supporting Physiotherapy and Exercise for People With Cystic Fibrosis in Low-Resource Settings.

Pediatric pulmonology·2026
Same author

Medical rehabilitation sits at the heart of rehabilitation services.

Advances in rehabilitation science and practice·2026
Same author

Development of a lifelong core outcome set for oesophageal atresia ± tracheoesophageal fistula: the OCELOT study.

BMJ open·2026
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Developmental Biology
  • Respiratory Physiology
  • Ion Channel Function

Background:

  • The cystic fibrosis transmembrane conductance regulator (CFTR) is a chloride channel crucial for various physiological processes.
  • Its precise role in embryonic lung development and airway function is not fully understood.
  • Investigating CFTR's impact on early lung development can elucidate its broader functions.

Purpose of the Study:

  • To determine the effect of CFTR knockout on embryonic lung growth.
  • To assess the impact of CFTR deficiency on airway peristalsis.
  • To examine airway smooth muscle distribution in the absence of CFTR.

Main Methods:

  • Utilized an established embryonic lung culture model.
  • Compared wild-type (cftr +/+) and knockout (cftr -/-) mouse embryos.

More Related Videos

Mouse Pneumonectomy Model of Compensatory Lung Growth
09:22

Mouse Pneumonectomy Model of Compensatory Lung Growth

Published on: December 17, 2014

Studying Wnt Signaling During Patterning of Conducting Airways
13:00

Studying Wnt Signaling During Patterning of Conducting Airways

Published on: October 16, 2016

Related Experiment Videos

Last Updated: Jun 27, 2026

Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching
07:32

Mouse Embryonic Lung Culture, A System to Evaluate the Molecular Mechanisms of Branching

Published on: June 30, 2010

Mouse Pneumonectomy Model of Compensatory Lung Growth
09:22

Mouse Pneumonectomy Model of Compensatory Lung Growth

Published on: December 17, 2014

Studying Wnt Signaling During Patterning of Conducting Airways
13:00

Studying Wnt Signaling During Patterning of Conducting Airways

Published on: October 16, 2016

  • Quantified lung area, perimeter, and lung bud count.
  • Assessed airway peristalsis frequency and mitotic cell percentages.
  • Evaluated airway smooth muscle distribution.

    • Main Results:

    • No significant differences were observed in lung area, perimeter, or lung bud count between wild-type and CFTR knockout embryos.
    • Airway peristalsis frequency and mitotic cell distribution in mesenchyme and epithelium were similar across genotypes.
    • Airway smooth muscle distribution appeared normal in all studied groups.

    Conclusions:

    • Embryonic lung growth is independent of CFTR function.
    • Airway peristalsis and smooth muscle distribution are not reliant on CFTR during embryonic development.
    • These findings suggest CFTR is not essential for normal embryonic lung development and basic airway functions.