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Related Experiment Video

Updated: Jun 27, 2026

Isolation, Processing and Analysis of Murine Gingival Cells
09:47

Isolation, Processing and Analysis of Murine Gingival Cells

Published on: July 2, 2013

Changes in MMPs and inflammatory cells in experimental gingivitis.

Márcio Lorencini1, Juliete A F Silva, Cristiane L R de la Hoz

  • 1Department of Microbiology and Immunology, State University of Campinas (UNICAMP), Campinas, SP, Brazil.

Histology and Histopathology
|December 17, 2008
PubMed
Summary
This summary is machine-generated.

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In early periodontal disease, matrix metalloproteinases (MMPs) like MMP-2 and MMP-9 are key drivers of extracellular matrix remodeling. Host inflammatory responses significantly contribute to the progression of gingivitis.

Area of Science:

  • Oral biology
  • Periodontology
  • Biochemistry

Background:

  • Periodontal disease involves extracellular matrix (ECM) disorganization, leading to tooth-periodontium adhesion loss.
  • Early stages show reduced collagen area but increased fiber compactness and thickness in gingiva.

Purpose of the Study:

  • Investigate the roles of matrix metalloproteinase-2 (MMP-2), MMP-9, MMP-14, and RECK in early ligature-induced gingivitis.
  • Elucidate the mechanisms behind ECM modifications during initial periodontal disease development.

Main Methods:

  • Gene expression analysis of MMPs and RECK.
  • Enzymatic activity assays for MMPs.
  • Immunohistochemistry to identify cell-specific MMP production.

Main Results:

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Related Experiment Videos

Last Updated: Jun 27, 2026

Isolation, Processing and Analysis of Murine Gingival Cells
09:47

Isolation, Processing and Analysis of Murine Gingival Cells

Published on: July 2, 2013

A Mouse Model for Pathogen-induced Chronic Inflammation at Local and Systemic Sites
09:52

A Mouse Model for Pathogen-induced Chronic Inflammation at Local and Systemic Sites

Published on: August 8, 2014

Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network
05:07

Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network

Published on: February 16, 2016

  • RECK gene expression remained unchanged.
  • MMP-14 expression decreased at 7 days; MMP-2 showed increased gene expression and activity early on.
  • MMP-9 peaked at 5 days, following its enzymatic activity peak at 2 days, correlating with immune cell influx.

Conclusions:

  • Extracellular matrix remodeling in early gingivitis is closely linked to MMP-2 and MMP-9 production and activation.
  • The host's inflammatory response is a critical factor in the advancement of periodontal disease.