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Updated: Jun 27, 2026
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An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis
Published on: October 4, 2018
Pathogenicity of infectious bronchitis virus isolates from Ontario chickens
Helena Grgić1, D Bruce Hunter, Peter Hunton
1Department of Pathobiology, Ontario Veterinary College, University of Guelph.
Summary
Five infectious bronchitis virus (IBV) isolates caused mild respiratory disease and reduced weight gain in chickens. Genetic analysis revealed relationships to known IBV strains, including a nephropathogenic variant.
Area of Science:
- Veterinary Virology
- Poultry Pathology
Background:
- Infectious bronchitis (IB) is a significant viral disease impacting commercial poultry globally.
- Despite vaccination protocols, IB remains a persistent challenge in Canadian poultry production.
Purpose of the Study:
- To assess the pathogenicity of five novel infectious bronchitis virus (IBV) isolates.
- To characterize the genetic relatedness of these IBV isolates to known strains.
Main Methods:
- Inoculation of specific pathogen-free (SPF) chickens with five IBV isolates.
- Clinical observation, gross pathology, and histological examination of infected chickens.
- S1 gene sequencing for phylogenetic analysis of IBV isolates.
Main Results:
- All five IBV isolates induced mild respiratory lesions in SPF chickens.
- Virus-inoculated chickens exhibited significantly reduced average daily weight gain during acute infection.
- Phylogenetic analysis grouped IBV-ON2, IBV-ON3, and IBV-ON5 with strain CU-82792.
- IBV-ON4 showed 98.7% identity to the nephropathogenic strain PA/1220/9.
Conclusions:
- The evaluated IBV isolates possess mild pathogenicity, primarily affecting respiratory health and growth performance.
- Genetic characterization highlights potential links to existing IBV lineages, including nephropathogenic variants.
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