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[Mechanism studies on hepatotoxicity of rats induced by fructus toosendan]
Shuang-yan Qi1, Ruo-min Jin, Hong-jie Liu
1Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Objective:
To study the effects on SOD, MDA, gamma-GT, GSH-Px and inflammatory factor (TNF-alpha, NF-kappaB, ICAM-1) in rats that induced by fructus toosendan, and to search for the hepatotoxicity mechanism of rats that induced by fructus toosendan.
Method:
The SD rats were given fructus toosendan 120 g x kg(-1) by orally for 45 days, then take the liver tissue of control and fructus toosendan group to prepare liver homogenate. The activities of SOD, the content of MDA, the ratio of SOD and MDA, the content of gamma-GT and glutathione peroxidase (GSH-Px) were detected according to the methods of kit. The tumor necosis factor-alpha (TNF-alpha) was detected by ABC-ELISA. The expression of NF-kappaB p65 and ICAM-1 were detected by immunohistochemistry.
Result:
The rats were given fructus toosendan 120 g x kg(-1) by orally for 45days, the SOD and GSH-Px activities in liver tissue decreased, the content of MDA increased, the ratio of SOD and MDA decreased, the content of gamma-GT and TNF-alpha, the masculine expression of NF-kappaB p65 and ICAM-1 increased.
Conclusion:
After the rats were given fructus toosendan, the liver can be damaged obviously, and the mechanism of hepatotoxicity perhaps related to free radical and inflammatory factor.
Insights
Fructus toosendan administration in rats significantly reduced antioxidant enzymes like superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), while increasing oxidative stress markers and inflammatory factors, indicating potential hepatotoxicity.
Area of Science:
- Biochemistry
- Toxicology
- Pharmacology
Context:
- The traditional use of Fructus toosendan necessitates understanding its potential toxicological effects.
- Hepatotoxicity is a significant concern for herbal medicines, requiring detailed mechanistic studies.
Purpose:
- To investigate the hepatotoxic effects of Fructus toosendan in a rat model.
- To elucidate the underlying mechanisms of Fructus toosendan-induced liver injury, focusing on oxidative stress and inflammation.
Summary:
- Rats orally administered Fructus toosendan (120 g x kg(-1)) for 45 days exhibited decreased liver superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities.
- Increased levels of malondialdehyde (MDA), gamma-glutamyl transpeptidase (gamma-GT), and tumor necrosis factor-alpha (TNF-alpha) were observed.
- Enhanced expression of NF-kappaB p65 and ICAM-1 further suggests an inflammatory response.
Impact:
- This study reveals that Fructus toosendan can cause significant liver damage in rats.
- The findings suggest that the hepatotoxicity mechanism involves oxidative stress and inflammatory pathways.
- Provides crucial data for assessing the safety profile of Fructus toosendan.
