Related Experiment Video

Updated: Jun 27, 2026

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
15:27

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Published on: April 17, 2017

Would you prefer multiple reaction monitoring or antibodies with your biomarker validation?

Haleem J Issaq, Timothy D Veenstra

    Expert Review of Proteomics
    |December 18, 2008
    PubMed
    Abstract

    No abstract available in PubMed .

    More Related Videos

    High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease09:00

    High Throughput Sequential ELISA for Validation of Biomarkers of Acute Graft-Versus-Host Disease

    Published on: October 31, 2012

    Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-1908:48

    Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

    Published on: February 16, 2022

    Related Concept Videos

    Enzyme-Linked Immunosorbent Assay01:33

    Enzyme-Linked Immunosorbent Assay

    In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
    There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.