Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interferon-gamma activates rat alveolar macrophages for anticryptococcal activity.

C H Mody1, C L Tyler, R G Sitrin

  • 1Department of Internal Medicine, University of Calgary, Alberta, Canada.

American Journal of Respiratory Cell and Molecular Biology
|July 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ASCEND: a randomised, double-blinded, placebo-controlled, phase II study of gemcitabine and nab-paclitaxel with LSTA1 in untreated metastatic pancreatic adenocarcinoma. An Australasian Gastro-Intestinal Trials Group (AGITG) trial.

ESMO gastrointestinal oncology·2026
Same author

A Scoping Review of Human Teratogens and Their Impact on the Developing Brain: A Contribution From the ConcePTION Project.

Birth defects research·2025
Same author

Patient Experiences of Using Wearable Health Monitors During Cancer Treatment: A Qualitative Study.

Clinical oncology (Royal College of Radiologists (Great Britain))·2024
Same author

Barriers to initiating and maintaining participation in parkrun.

BMC public health·2022
Same author

Informing the public health response to COVID-19: a systematic review of risk factors for disease, severity, and mortality.

BMC infectious diseases·2021
Same author

The first case of HIV-2 in Scotland.

Access microbiology·2020

Interferon-gamma (IFN-gamma) activates rat alveolar macrophages (AM) to fight the yeast Cryptococcus neoformans. This immune response is crucial for host defense against fungal infections, especially in immunocompromised individuals.

Area of Science:

  • Immunology
  • Mycology
  • Cell Biology

Background:

  • Cryptococcus neoformans is a yeast pathogen primarily affecting immunosuppressed individuals.
  • Alveolar macrophages (AM) are key pulmonary immune cells defending against C. neoformans.
  • The activation of rat AM for enhanced anticryptococcal activity by immune products was previously uncharacterized.

Purpose of the Study:

  • To investigate the in vitro activation of rat AM for enhanced anticryptococcal activity.
  • To determine the role of interferon-gamma (IFN-gamma) in this activation process.
  • To explore the potential therapeutic implications of lymphokine administration in cryptococcal infections.

Main Methods:

  • Rat AM were incubated with medium conditioned by concanavalin A-stimulated splenic lymphocytes.

Related Experiment Videos

  • Monoclonal antibodies neutralizing IFN-gamma were used to assess its role.
  • Recombinant IFN-gamma and lipopolysaccharide were applied to evaluate their effects on AM activity.
  • Enzyme-linked immunosorbent assay (ELISA) quantified IFN-gamma levels.

    • Main Results:

    • Lymphokine-conditioned medium (LCM) activated rat AM for anticryptococcal activity.
    • IFN-gamma was identified as necessary and sufficient for LCM-induced AM activation.
    • Recombinant IFN-gamma mimicked the activating effects of LCM.
    • Both LCM and IFN-gamma enhanced macrophage association with C. neoformans.

    Conclusions:

    • IFN-gamma is a critical mediator for activating alveolar macrophages against Cryptococcus neoformans.
    • Deficiencies in IFN-gamma production may contribute to susceptibility in immunosuppressed hosts.
    • Therapeutic administration of IFN-gamma could potentially enhance cell-mediated immunity against fungal infections.