Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular origin of viscoelastic transition in molecular granular materials: insights from molecular dynamics simulations.

Physical chemistry chemical physics : PCCP·2026
Same author

Chemically-fueled transient peptide hydrogel enabling programmable time-gated functions.

Chemical communications (Cambridge, England)·2026
Same author

Accurate detection of tumor clonality and ongoing expansion mode from genomic data.

bioRxiv : the preprint server for biology·2026
Same author

Analysis of Fall and Jump Behaviors in Freely Moving <i>Drosophila melanogaster</i> Using 58 fps Video.

Insects·2026
Same author

Valence-Controlled Flexibility and Gelation in Cerium-Polyoxometalate Subnanowires.

Nano letters·2026
Same author

Single-nucleus multiome sequencing identifies candidate regulators of mouse gastric epithelial homeostasis.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Jun 27, 2026

Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers
07:39

Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers

Published on: June 4, 2019

Simultaneous tracking of fly movement and gene expression using GFP.

Dhruv Grover1, Junsheng Yang, Simon Tavaré

  • 1Molecular and Computational Biology Program, Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089-2910, USA. dhruvgro@usc.edu

BMC Biotechnology
|December 18, 2008
PubMed
Summary
This summary is machine-generated.

This study introduces a new system to track Green Fluorescent Protein (GFP) expression and correlate it with fly movement. The system reveals circadian gene patterns and expression spikes before death, offering new insights into fly aging and behavior.

More Related Videos

Multiplex Detection of Gene Expression in the Intact Drosophila Brain Using Expansion-Assisted Iterative Fluorescence In Situ Hybridization
09:05

Multiplex Detection of Gene Expression in the Intact Drosophila Brain Using Expansion-Assisted Iterative Fluorescence In Situ Hybridization

Published on: May 2, 2025

High-Resolution Video Tracking of Locomotion in Adult Drosophila Melanogaster
09:08

High-Resolution Video Tracking of Locomotion in Adult Drosophila Melanogaster

Published on: February 20, 2009

Related Experiment Videos

Last Updated: Jun 27, 2026

Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers
07:39

Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers

Published on: June 4, 2019

Multiplex Detection of Gene Expression in the Intact Drosophila Brain Using Expansion-Assisted Iterative Fluorescence In Situ Hybridization
09:05

Multiplex Detection of Gene Expression in the Intact Drosophila Brain Using Expansion-Assisted Iterative Fluorescence In Situ Hybridization

Published on: May 2, 2025

High-Resolution Video Tracking of Locomotion in Adult Drosophila Melanogaster
09:08

High-Resolution Video Tracking of Locomotion in Adult Drosophila Melanogaster

Published on: February 20, 2009

Area of Science:

  • Molecular Biology
  • Chronobiology
  • Animal Behavior

Background:

  • Green Fluorescent Protein (GFP) is widely used for transgene expression monitoring.
  • GFP has limitations in tracking circadian gene expression and correlating it with animal behavior.
  • Previous methods could not link reporter gene expression to 3D movement in real-time.

Purpose of the Study:

  • To develop a novel video tracking system for real-time quantification of tissue-specific GFP expression.
  • To correlate GFP reporter patterns with 3D animal movement and behavior.
  • To investigate circadian gene expression and its relationship with aging and mortality.

Main Methods:

  • Developed a video tracking system for simultaneous monitoring of GFP expression and animal movement.
  • Utilized tissue-specific GFP reporters (eyeless/Pax6, hsp70, hsp22) in Drosophila.
  • Quantified reporter gene expression and correlated it with fly activity and lifespan.

Main Results:

  • The system successfully correlated GFP expression with 3D fly movement in real-time.
  • eyeless/Pax6 reporter showed a 12-hour circadian period linked to fly activity.
  • hsp70 and hsp22 reporters exhibited circadian patterns during aging, with expression spikes preceding death.

Conclusions:

  • GFP is effective for longitudinal assessment of fly movement and tissue-specific gene expression.
  • hsp-GFP reporters accurately reflect endogenous gene patterns, including circadian periodicity and aging-induced expression.
  • The study revealed pre-mortem expression spikes and significant fly-to-fly variability in circadian gene expression phases.