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Biotin homeostasis during the cell cycle.

J Zempleni1, D M Mock

  • 1Department of Nutritional Science & Dietetics, University of Nebraska-Lincoln, 316 Ruth Leverton Hall, Lincoln, NE 68583, USA.

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|December 18, 2008
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Summary
This summary is machine-generated.

Peripheral blood mononuclear cells (PBMC) show increased biotin uptake during proliferation, suggesting a higher demand for this vitamin. This enhanced uptake is linked to specific gene expression changes and histone biotinylation, potentially impacting DNA replication and transcription.

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Area of Science:

  • Cellular Biology
  • Nutritional Biochemistry
  • Molecular Biology

Background:

  • Peripheral blood mononuclear cells (PBMC) utilize a Na-dependent, energy-requiring transporter for biotin accumulation.
  • Kinetic data suggests the presence of a specific biotin transporter beyond the Na-dependent multivitamin transporter.
  • Cell proliferation in PBMC is associated with increased biotin uptake.

Purpose of the Study:

  • To investigate the mechanisms and implications of increased biotin uptake in proliferating PBMC.
  • To explore the relationship between biotin demand and gene expression in cellular proliferation.
  • To examine the role of biotin in histone modification during DNA replication and transcription.

Main Methods:

  • Analysis of biotin transporter kinetics in PBMC.
  • Measurement of biotin uptake in proliferating versus non-proliferating PBMC.
  • Gene expression analysis for biotin-dependent carboxylases and holocarboxylase synthetase.
  • Assessment of histone biotinylation levels in proliferating PBMC.

Main Results:

  • Proliferating PBMC exhibit increased biotin uptake, mediated by an increased number of cell surface transporters.
  • PBMC increase the expression of genes encoding biotin-dependent carboxylases (beta-methylcrotonyl-CoA carboxylase, propionyl-CoA carboxylase) and holocarboxylase synthetase.
  • Increased biotinylation of histones is observed in proliferating PBMC, suggesting a role in DNA processes.

Conclusions:

  • Proliferating PBMC have a significantly increased demand for biotin, driven by the synthesis of new carboxylases.
  • The observed changes in biotin transport and utilization support a role for biotin in cellular proliferation.
  • Biotinylation of histones in proliferating cells suggests a potential involvement in DNA transcription and replication.