Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Protein Metabolism01:21

Overview of Protein Metabolism

Proteins are broken down into amino acids during digestion. Unlike fats and carbohydrates, which are stored for later use, proteins are not. Instead, amino acids are either used to produce ATP through oxidation or contribute to the creation of new proteins for the growth and repair of the body. Any surplus amino acids from the diet are converted into glucose or triglycerides rather than excreted.
Amino acids play various roles in the body once they are absorbed into cells. They are restructured...
Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Phosphorylation01:02

Phosphorylation

The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
Protein Digestion01:02

Protein Digestion

Protein digestion begins in the stomach, where the highly acidic environment can easily disrupt protein structure by exposing the peptide bonds of polypeptide chains. After polypeptide chains are broken into individual amino acids by a series of digestive enzymes, the amino acids are transported to the liver via the bloodstream to produce energy.
Protein Denaturation01:28

Protein Denaturation

The function of proteins depends on their native three-dimensional structure, which is dictated by the amino acid sequence of the specific protein. Folding of the polypeptide chain takes place under specific conditions that energetically favor the folded conformation. In contrast, protein denaturation occurs spontaneously under unfavorable conditions that disrupt the integrity of the folded conformation. Thus, the chemical and physical environment of a protein, such as significant changes in pH...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First Search for Axion Dark Matter with a MADMAX Prototype.

Physical review letters·2025
Same author

Search for the in-situ production of <math><mmultiscripts><mrow></mrow> <mrow></mrow> <mn>77</mn></mmultiscripts></math> Ge in the GERDA neutrinoless double-beta decay experiment.

The European physical journal. C, Particles and fields·2025
Same author

Measurement of the <sup>85</sup>Kr specific activity in the GERDA liquid argon.

The European physical journal. C, Particles and fields·2025
Same author

First Search for Dark Photon Dark Matter with a madmax Prototype.

Physical review letters·2025
Same author

Experimental Observation of the Motion of Ions in a Resonantly Driven Plasma Wakefield Accelerator.

Physical review letters·2025
Same author

Emerging insights in tropical rheumatology: Challenges, clinical manifestations, and recent advances.

Best practice & research. Clinical rheumatology·2025

Related Experiment Video

Updated: Jun 27, 2026

Identification of Post-translational Modifications of Plant Protein Complexes
10:07

Identification of Post-translational Modifications of Plant Protein Complexes

Published on: February 22, 2014

Phytate and phytase: consequences for protein utilisation.

P H Selle1, V Ravindran, A Caldwell

  • 1BASF Animal Nutrition, 87a St Hilliers Road, Auburn, NSW 2144, Australia.

Nutrition Research Reviews
|December 18, 2008
PubMed
Summary

Microbial phytase enzymes improve nutrient utilization in pigs and poultry by reducing phytate, a compound in plant-based feeds that hinders phosphorus and protein absorption. Further research is needed to fully understand these protein-phytate interactions.

More Related Videos

Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases
09:07

Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases

Published on: December 23, 2022

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis
05:47

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

Published on: June 25, 2020

Related Experiment Videos

Last Updated: Jun 27, 2026

Identification of Post-translational Modifications of Plant Protein Complexes
10:07

Identification of Post-translational Modifications of Plant Protein Complexes

Published on: February 22, 2014

Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases
09:07

Concanavalin A-Based Sedimentation Assay to Measure Substrate Binding of Glucan Phosphatases

Published on: December 23, 2022

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis
05:47

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

Published on: June 25, 2020

Area of Science:

  • Animal Nutrition
  • Biochemistry
  • Environmental Science

Background:

  • Intensive pig and poultry farming leads to significant phosphorus (P) excretion due to poor phytate-bound P availability in plant-based feeds.
  • This environmental issue drove the adoption of microbial phytase feed enzymes for monogastric animals.
  • Phytate is now recognized for negatively impacting protein utilization, not just P availability.

Purpose of the Study:

  • To review the nutritional relevance of protein-phytate interactions in pigs and poultry.
  • To summarize the effects of microbial phytases on amino acid digestibility and utilization.
  • To discuss potential mechanisms behind phytate's negative effects on protein.

Main Methods:

  • Literature review focusing on studies involving microbial phytase supplementation.
  • Analysis of research on amino acid digestibility and utilization in response to phytase.
  • Discussion of proposed mechanisms for protein-phytate interactions.

Main Results:

  • Microbial phytases enhance amino acid digestibility and utilization in pigs and poultry.
  • Potential mechanisms include releasing protein from complexes, preventing complex formation, improving digestive enzyme function, and reducing endogenous amino acid losses.
  • Responses to phytase can be variable.

Conclusions:

  • Understanding protein-phytate interactions and phytase mechanisms requires further investigation.
  • Factors influencing variable responses to phytase include feed ingredient properties and phytase efficacy in different diets.
  • More research is needed to quantify these factors and optimize phytase application.