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Distinctive autofluorescence of urine samples from individuals with bacteriuria compared with normals
Ayad G Anwer1, Perinchery M Sandeep, Ewa M Goldys
1Department of Biomedical Applications, Institute of Laser for Postgraduate Studies, University of Baghdad, Iraq.
Background:
A variety of fluorophores are present in normal human urine. Alteration in the autofluorescence of urine could result from physiological or pathological changes.
Method:
This study investigates the differences in the autofluorescence of 45 normal urine samples from 25 individuals with bacteriuria.
Results:
Excitation at 290 nm showed good discrimination between these 2 groups. Principal Component Analysis (PCA) of the data revealed statistically significant differences between the fluorescence spectra for samples with bacteriuria as compared to the control group.
Conclusion:
The findings indicate the potential of the fluorescence spectrum of urine to be developed as a simple and rapid diagnostic tool.
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