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Related Experiment Video

Updated: Jun 27, 2026

Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test
05:25

Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test

Published on: July 14, 2023

Antibody discovery via multiplexed single cell characterization.

William D Harriman1, Ellen J Collarini, Gizette V Sperinde

  • 1Trellis Bioscience, 2-B Corporate Dr., South San Francisco, CA 94080, United States.

Journal of Immunological Methods
|December 18, 2008
PubMed
Summary
This summary is machine-generated.

This study introduces a novel method for identifying specific antibody-producing hybridoma cells. The technique uses fluorescent beads to simultaneously screen for multiple antibody properties, enabling efficient isolation of rare cell populations.

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Area of Science:

  • Biotechnology
  • Immunology
  • Cell Biology

Background:

  • Hybridoma technology is crucial for monoclonal antibody production.
  • Screening large hybridoma populations for specific antibody characteristics is challenging.
  • Current methods often lack the multiplexing capability to assess multiple antibody properties simultaneously.

Purpose of the Study:

  • To develop a high-throughput method for screening hybridoma cells based on secreted antibody properties.
  • To enable concurrent assessment of antibody specificity and affinity in a primary screen.
  • To efficiently isolate rare hybridoma cells with desired antibody profiles.

Main Methods:

  • Utilized functionalized fluorescent latex beads for multiplexed detection of antibody footprints.
  • Developed a system to probe up to 9 antibody properties concurrently from single hybridoma cells.
  • Implemented a method for plating cells on a membrane to capture secreted antibodies for analysis.
  • Integrated cell immobilization and antibody footprint analysis for cell recovery.

Main Results:

  • Successfully screened single hybridoma cells for multiple antibody characteristics, including specificity and affinity.
  • Demonstrated the ability to rank antibody-binding avidity by modulating antigen density on beads.
  • Achieved efficient isolation of rare hybridoma cells, with positive cells found at frequencies below 1/50,000.
  • Validated the recovery and subsequent cloning of desired hybridoma cells.

Conclusions:

  • The developed method offers a powerful tool for selecting high-affinity, specific antibody-producing hybridoma cells.
  • This approach significantly enhances the efficiency of hybridoma screening and cell line development.
  • The technique is applicable for isolating rare cells based on complex secreted product profiles.