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Related Experiment Video

Updated: Jun 27, 2026

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

Published on: November 4, 2018

The co-chaperone BAG3 regulates Herpes Simplex Virus replication.

Christos A Kyratsous¹, Saul J Silverstein

¹Department of Microbiology, College of Physicians and Surgeons, Columbia University, 701 West 168th Street, New York, NY 10032, USA.

Proceedings of the National Academy of Sciences of the United States of America

|December 18, 2008

Summary

This study identifies BAG3 as a key co-chaperone regulating herpes virus gene expression and replication. Herpes simplex virus replication is impaired in cells lacking BAG3, highlighting its crucial role in viral processes.

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Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

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Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus

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Published on: October 7, 2011

Area of Science:

Virology
Molecular Biology
Cellular Biology

Background:

Viruses rely on host cell machinery for replication.
Cellular proteins are essential for various stages of the viral life cycle.

Purpose of the Study:

To identify host cell proteins regulating herpes virus gene expression.
To investigate the role of BAG3 in herpes simplex virus replication.

Main Methods:

Gene silencing of BAG3 in host cells.
Analysis of herpes simplex virus replication and gene expression.
Investigating the role of ICP0 and promyelocytic leukemia.

Main Results:

BAG3 acts as a regulator of herpes virus immediate early gene expression.
Herpes simplex virus lacking ICP0 shows reduced replication in BAG3-silenced cells.
BAG3 enhances virus gene expression and increases herpes simplex virus replication independently of promyelocytic leukemia.

Conclusions:

BAG3 is a critical host co-chaperone for herpes simplex virus replication.
BAG3 plays a significant role in augmenting viral gene expression and replication efficiency.