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Updated: Jun 27, 2026

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins
Published on: August 31, 2017
Upstream-binding factor is sequestered into herpes simplex virus type 1 replication compartments
Nigel D Stow1, Vanessa C Evans, David A Matthews
1MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK.
Abstract:
Previous reports have shown that adenovirus recruits nucleolar protein upstream-binding factor (UBF) into adenovirus DNA replication centres. Here, we report that despite having a different mode of viral DNA replication, herpes simplex virus type 1 (HSV-1) also recruits UBF into viral DNA replication centres. Moreover, as with adenovirus, enhanced green fluorescent protein-tagged fusion proteins of UBF inhibit viral DNA replication. We propose that UBF is recruited to the replication compartments to aid replication of HSV-1 DNA. In addition, this is a further example of the role of nucleolar components in viral life cycles.
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