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Related Concept Videos

Herpes01:28

Herpes

Herpes simplex type 1 (HSV‑1) is a widespread pathogen responsible for orolabial lesions. It is an enveloped, double-stranded DNA (dsDNA) virus belonging to the family Herpesviridae. Once the virus infects a host cell, its double‑stranded DNA genome is delivered into the nucleus, where a coordinated cascade of immediate‑early, early, and late gene expression directs viral DNA replication, structural protein synthesis, and virion assembly. After primary infection of epithelial cells, HSV-1...
Viruses with RNA Genomes01:29

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
Inhibitors Of Virion Release01:25

Inhibitors Of Virion Release

Viral replication and dissemination rely on efficient mechanisms for host cell entry, genome replication, assembly, and release. Influenza viruses, such as types A and B, are negative-sense single-stranded RNA viruses with a segmented genome, that depend on two critical surface glycoproteins to carry out these processes: hemagglutinin (HA) and neuraminidase (NA). HA initiates infection by binding to sialic acid residues on the surface of host epithelial cells, facilitating receptor-mediated...
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
Retrovirus Life Cycles01:10

Retrovirus Life Cycles

Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the retrovirus to...

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Related Experiment Video

Updated: Jun 27, 2026

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins
08:26

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Published on: August 31, 2017

Upstream-binding factor is sequestered into herpes simplex virus type 1 replication compartments.

Nigel D Stow1, Vanessa C Evans, David A Matthews

  • 1MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, UK.

The Journal of General Virology
|December 18, 2008
PubMed
Summary
This summary is machine-generated.

Herpes simplex virus type 1 (HSV-1) recruits nucleolar protein upstream-binding factor (UBF) to its replication centers, similar to adenovirus. UBF

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Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins
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Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy
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Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

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Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry
09:38

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

Published on: June 26, 2019

Area of Science:

  • Virology
  • Molecular Biology
  • Cell Biology

Background:

  • Adenovirus is known to recruit nucleolar protein upstream-binding factor (UBF) to its DNA replication centers.
  • The role of nucleolar components in viral life cycles is an emerging area of research.

Purpose of the Study:

  • To investigate whether herpes simplex virus type 1 (HSV-1) also recruits UBF into viral DNA replication centers.
  • To determine the functional significance of UBF recruitment in HSV-1 DNA replication.

Main Methods:

  • Utilizing techniques to visualize the localization of UBF during HSV-1 infection.
  • Employing methods to assess the impact of UBF on viral DNA replication, including the use of UBF-fusion proteins.

Main Results:

  • HSV-1, similar to adenovirus, recruits UBF into its viral DNA replication centers.
  • Expression of enhanced green fluorescent protein-tagged UBF fusion proteins inhibits HSV-1 DNA replication.

Conclusions:

  • UBF is recruited to HSV-1 replication compartments, suggesting a role in aiding viral DNA replication.
  • This finding highlights a conserved mechanism involving nucleolar components in the life cycles of different viruses, including HSV-1.