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Variants of open reading frame Bm126 in wild-type Bombyx mori nucleopolyhedrovirus isolates exhibit functional

Bifang Hao1, Jinshan Huang, Xiulian Sun

  • 1Northwest A&F University, Yangling, Shaanxi 712100, PR China.

The Journal of General Virology
|December 18, 2008
PubMed

Insights

The Bombyx mori nucleopolyhedrovirus (BmNPV) open reading frame 126 (Bm126) gene deletion delayed larval survival time. Repairing the deletion with Bm126-SX rescued this delay, while Bm126-GD increased occlusion body yield.

Area of Science:

  • Virology
  • Molecular Biology
  • Insect Pathology

Background:

  • The Bombyx mori nucleopolyhedrovirus (BmNPV) open reading frame (ORF) 126 (Bm126) is a member of the baculovirus 11K protein family.
  • Bm126 homologues are known in other baculoviruses, suggesting conserved functions.

Purpose of the Study:

  • To investigate the function of Bm126 in BmNPV infection.
  • To characterize different subtypes of Bm126 found in Chinese isolates.

Main Methods:

  • Amplification and sequence analysis of Bm126 from different BmNPV isolates.
  • Construction and characterization of Bm126-knockout and repaired bacmids.
  • Assessment of viral infectivity, larval survival time, and occlusion body (OB) yield.

Main Results:

  • Two Bm126 subtypes, Bm126-SX and Bm126-GD, were identified and differed from the BmNPV T3 isolate.
  • Deletion of Bm126 delayed larval survival time but did not affect budded virus growth or lethal dose of OBs.
  • Repairing the Bm126 deletion with Bm126-SX rescued the delayed survival, while Bm126-GD repair significantly increased OB yield.

Conclusions:

  • Bm126 plays a role in modulating BmNPV infection dynamics, specifically affecting larval survival time.
  • Different Bm126 subtypes exhibit distinct functional properties, with Bm126-SX involved in survival and Bm126-GD in enhancing OB production.

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