Related Experiment Video
Updated: Jun 27, 2026

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
Published on: December 23, 2017
Identification of sequence elements regulating promoter activity and replication of a monopartite
Omid Eini1, S A Akbar Behjatnia, Satish Dogra
1School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, SA 5064, Australia.
Abstract:
DNA beta is a circular single-stranded satellite DNA associated with certain monopartite begomoviruses (family Geminiviridae) which causes economically important diseases such as cotton leaf curl disease. DNA beta contains a single gene, betaC1, which encodes a pathogenicity protein responsible for symptom production. Transient expression studies in Nicotiana tabacum using the beta-glucuronidase reporter gene driven by a betaC1 promoter-deletion series of the DNA beta associated with cotton leaf curl Multan virus identified a 68 nt region (between -139 and -207) which is important for betaC1 transcription. This 68 nt region contains a G-box (CACGTG) located 143 nt upstream of the betaC1 start codon. Mutation of the G-box resulted in a significant reduction in betaC1 promoter activity and DNA beta replication efficiency. In addition, the G-box motif was found to bind specifically to a protein(s) in nuclear extracts prepared from tobacco leaf tissues. Our results indicate that interaction of the G-box motif with host nuclear factors is important for efficient gene expression and replication of DNA beta.
Related Concept Videos
Subviral Agents
The Eukaryotic Promoter Region
The Eukaryotic Promoter Region
DNA-only Transposons
The donor site from where the transposon is excised is either degraded or...
DNA Bacteriophages
Reporter Genes
Commonly used reporter...

