Related Experiment Video
Updated: Jun 27, 2026
Advancements in Bovine Organoid Technology Using Small and Large Intestinal Monolayer Interfaces
Published on: June 14, 2024
Unidirectional transport across cultured ovine amniotic epithelial cell monolayer
Cecilia Y Cheung1, Robert A Brace
1Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Oregon Health and Science University, Portland, Oregon 97210, USA.
Objective:
To determine whether ovine amniotic cells in monolayer culture exhibit unidirectional transport.
Methods:
Permeability of (14)C-inulin was measured in two directions across ovine amniotic cells grown to confluence on cell culture inserts.
Results:
Permeability was 0.69 + 0.17 (SE) microl/min/cm(2) from the top of the insert where the cells were attached to the bottom and 0.11 + 0.02 microl/min/cm(2) in the reverse direction (P < 0.001). Permeability was similar in the two directions for chorionic cells and umbilical vein endothelial cells. Addition of vascular endothelial growth factor did not alter permeability.
Conclusions:
Transcellular transport in ovine amniotic monolayers is directionally specific. The transport ratio of 6.2:1 for (14)C-inulin is comparable to the in vivo ratio of 4.7:1 for the ovine intramembranous pathway and is consistent with passive diffusion in both directions and vesicular transport in one direction across the monolayer at 5.2 times the rate of passive diffusion.
More Related Videos
07:56Generation of a Bovine Primary Enteroid-Derived Two-Dimensional Monolayer Culture System for Applications in Translational Biomedical Research
Published on: April 5, 2024
10:00In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
Published on: November 28, 2019