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Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase
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[7-aminoactinomycin as a fluorescent probe for DNA unwinding and denaturation]

M A Bitekhtina1, N L Vekshin

  • 1Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290 Russia.

Bioorganicheskaia Khimiia
|December 18, 2008
PubMed

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...