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Updated: Jun 27, 2026

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
Published on: May 21, 2015
Presence of bacteria in dentinal tubules
José Ricardo Kina1, Juliana Kina, Eunice Fumico Umeda Kina
1Division of Periodontics, Department of Surgery and Integrated Clinic, Dental School of Araçatuba, São Paulo State University, Araçatuba, SP, Brazil. kinajr@hotmail.com
Abstract:
This study demonstrated that a significant number of bacteria is present in the radicular dentinal tubules of periodontally diseased human teeth. Ten periodontally diseased teeth were prepared and stained by Brown and Brenn technique for histological examination. Bacteria were detected in all teeth. It is suggested that bacteria may invade dentinal tubules exposed to periodontal pocket and are very hard to be eliminated by conventional mechanical and chemical periodontal therapy. Contaminated dentinal tubules of periodontally diseased teeth can thus act as active bacterial reservoirs to promote recolonization of mechanically treated root surfaces, which could interfere with the periodontal healing and progression of the disease.
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