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Urinary PGE2 in rats with chronic partial unilateral ureteral obstruction
B R Sankari1, G F Steinhardt, L Salinas-Madrigal
1Department of Surgery, St. Louis University School of Medicine, Missouri 63104.
The Journal of Surgical Research
|September 1, 1991
Summary
Chronic partial unilateral ureteral obstruction (UUO) in rats significantly reduces urinary prostaglandin E2 (PGE2) production. This impaired PGE2 elaboration occurs in the obstructed kidney and affects its ability to synthesize this key inflammatory mediator.
Area of Science:
- Nephrology
- Urology
- Biochemistry
Background:
- Prostaglandin E2 (PGE2) plays a crucial role in renal function and inflammation.
- Unilateral ureteral obstruction (UUO) is a model used to study kidney injury and disease progression.
Purpose of the Study:
- To investigate the impact of chronic partial UUO on urinary PGE2 levels and renal PGE2 synthesis in rats.
Main Methods:
- Measurement of urinary PGE2 in rats with surgically induced chronic partial UUO and sham-operated controls.
- Collection and analysis of urine from cannulated ureters to assess PGE2 excretion.
- Determination of PGE2 synthesis in isolated renal medullary and cortical slices, with and without arachidonic acid stimulation.
Main Results:
- Bladder urine PGE2 levels were significantly lower in UUO rats compared to sham controls.
- The obstructed kidney in UUO rats showed reduced PGE2 excretion compared to the contralateral kidney.
- Renal medullary slices from obstructed kidneys synthesized less PGE2 and failed to respond to arachidonic acid stimulation, unlike contralateral kidney slices.
Conclusions:
- Chronic partial UUO in rats leads to a significant decrease in PGE2 elaboration.
- The obstructed kidney exhibits impaired PGE2 synthesis capacity, suggesting a role for reduced PGE2 in the pathophysiology of UUO-induced kidney damage.