Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Association study of APC polymorphisms with colorectal cancer in Han Chinese.

Clinical biochemistry·2012

Same author

Label-free quantitative proteomic analysis reveals dysfunction of complement pathway in peripheral blood of schizophrenia patients: evidence for the immune hypothesis of schizophrenia.

Molecular bioSystems·2012

Same author

A role for c-Abl in cell senescence and spontaneous immortalization.

Age (Dordrecht, Netherlands)·2012

Same author

Genome-wide association study in Han Chinese identifies four new susceptibility loci for coronary artery disease.

Nature genetics·2012

Same author

c-Abl promotes osteoblast expansion by differentially regulating canonical and non-canonical BMP pathways and p16INK4a expression.

Nature cell biology·2012

Same author

Dynamic network of transcription and pathway crosstalk to reveal molecular mechanism of MGd-treated human lung cancer cells.

PloS one·2012

Search research articles

Related Experiment Video

Updated: Dec 23, 2025

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

Published on: November 9, 2017

Particle stability in polymer-assisted reverse colorimetric DNA assays.

Weiming Zheng¹, Lin He

¹Department of Chemistry, North Carolina State University, Raleigh, NC 27695, USA.

Analytical and Bioanalytical Chemistry

|December 18, 2008

Summary

This study details a reverse colorimetric DNA detection method. Optimized conditions prevent gold nanoparticle aggregation, ensuring assay stability and accurate colorimetric results.

More Related Videos

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

Published on: October 6, 2017

Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples

Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples

Published on: February 4, 2013

Related Experiment Videos

Last Updated: Dec 23, 2025

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

Published on: November 9, 2017

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

Published on: October 6, 2017

Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples

Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples

Published on: February 4, 2013

Area of Science:

Nanotechnology
Analytical Chemistry
Biochemistry

Background:

Colorimetric assays offer simple detection methods.
Existing assays can suffer from nanoparticle aggregation issues.
Developing robust nanoparticle-based detection is crucial.

Purpose of the Study:

To investigate the reaction kinetics of polymer growth and particle aggregation in a reverse colorimetric DNA assay.
To optimize conditions for stable nanoparticle-based DNA detection.
To understand the role of copper ions in the assay.

Main Methods:

Utilized core-shell particle formation upon DNA hybridization.
Covalently linked polymer initiators to nanoparticles.
Analyzed polymer chain growth and particle aggregation kinetics.
Investigated the influence of copper ion concentration, salt concentration, and temperature.

Main Results:

DNA hybridization triggers polymer shell formation, preventing aggregation.
Copper ions facilitate both polymerization and unwanted particle aggregation.
Assay performance is sensitive to copper ion concentration, salt, and temperature.
Optimized conditions favor polymer growth over aggregation, ensuring assay stability.

Conclusions:

The reverse colorimetric DNA assay relies on controlled polymer shell formation.
Understanding and controlling copper ion interactions is key to assay robustness.
Optimized steric stabilization via polymer growth ensures reliable colorimetric DNA detection.