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Published on: September 28, 2013
Electrophoretic analysis of total protein profiles of some Lathyrus L. (Sect. Cicercula) grown in Turkey
Irfan Emre1, Dilek Turgut-Balik, Ahmet Sahin
1Department of Primary Education, Faculty of Education, Firat University, Elazig, Turkey.
Abstract:
In this study, total 8 species (Section Cicercula) belong to 14 different geographic distributions collected from Turkey have been studied for the analysis of seed storage protein profiles to examine their relationship by Sodium Dodecyl Sulphate Polyacrilamide Gel Electrophoresis (SDS-PAGE) technique. Hierarchical cluster analysis and Euclidean distance were used for comparison between species and calculating the genetic resemblance respectively. Dendogram was formed using average linkage. Electrophoretic protein profiles of seed cotyledons were showed that all species formed two clusters. The first one consisted of the L. stenophyllus, L. hirsutus, L. chloranthus, L. cicera and L. sativus second one by three species (L. annuus, L. cassius and L. phaselitanus). In addition, very little differences were observed in total protein profiles of the examined sample species (L. cicera, L. hirsutus and L. chloranthus) from nine geographical regions belong to section Cicercula. Protein amount was found to be highest in L. hirsutus and lowest in L. cicera.
