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Detection of Ureaplasma urealyticum in semen of infertile men by PCR

Habib Zeighami1, Shahin Najar Peerayeh, Maryam Safarlu

  • 1Young Researchers Club, Azad University, Iran.

Pakistan Journal of Biological Sciences : PJBS
|December 19, 2008
PubMed

Insights

Ureaplasma urealyticum infections are more prevalent in infertile men, impacting semen quality. This bacterium, linked to infertility, was detected in 12% of infertile men

Area of Science:

  • Microbiology
  • Reproductive Medicine
  • Molecular Biology

Background:

  • Ureaplasma urealyticum is a known cause of genitourinary infections.
  • The organism is frequently found in infertile couples and may affect fertility outcomes.
  • U. urealyticum poses risks to fertility treatments and pregnancy.

Purpose of the Study:

  • To investigate the prevalence of Ureaplasma urealyticum in the semen of infertile men compared to healthy controls.
  • To assess the correlation between U. urealyticum infection and semen parameters in infertile men.

Main Methods:

  • Semen samples were collected from infertile patients and healthy controls.
  • Samples underwent routine andrological analysis and Polymerase Chain Reaction (PCR) for U. urealyticum detection.
  • DNA extraction utilized the Cadieux method, with species-specific primers targeting the urease gene.

Main Results:

  • Ureaplasma urealyticum was detected in 12% of infertile men and 3% of healthy controls via PCR.
  • Infertile men exhibited significantly reduced semen volume, sperm concentration, and normal sperm morphology.
  • Infertile men positive for U. urealyticum showed poorer semen parameters than PCR-negative infertile men.

Conclusions:

  • Ureaplasma urealyticum is significantly more prevalent in infertile men.
  • U. urealyticum infection is associated with decreased semen quality in infertile individuals.
  • The findings highlight the potential role of U. urealyticum in male infertility.

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