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Related Concept Videos

Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.

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Related Experiment Video

Updated: Jun 27, 2026

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation
07:40

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation

Published on: July 31, 2015

Mechanically enhanced microcapsules for cellular gene therapy.

F Shen1, M A J Mazumder, N A D Burke

  • 1Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada.

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
|December 19, 2008
PubMed
Summary
This summary is machine-generated.

New microcapsules for gene therapy feature a covalently cross-linked coating, significantly improving stability over traditional alginate-poly(L-lysine)-alginate capsules. These enhanced capsules show promise as immunoisolation devices with maintained biocompatibility and permeability.

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Encapsulated Cell Technology for the Delivery of Biologics to the Mouse Eye
06:10

Encapsulated Cell Technology for the Delivery of Biologics to the Mouse Eye

Published on: March 30, 2020

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Last Updated: Jun 27, 2026

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation
07:40

Gene Transfection toward Spheroid Cells on Micropatterned Culture Plates for Genetically-modified Cell Transplantation

Published on: July 31, 2015

Encapsulated Cell Technology for the Delivery of Biologics to the Mouse Eye
06:10

Encapsulated Cell Technology for the Delivery of Biologics to the Mouse Eye

Published on: March 30, 2020

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Gene Therapy

Background:

  • Alginate-poly(L-lysine)-alginate (APA) microcapsules offer ionic cross-linking for cellular gene therapy.
  • Limitations exist in the stability and robustness of ionically cross-linked microcapsules.

Purpose of the Study:

  • To develop and evaluate microcapsules with a covalently cross-linked coating for enhanced stability in cellular gene therapy.
  • To compare the stability, permeability, and biocompatibility of novel microcapsules against traditional APA microcapsules.

Main Methods:

  • Fabrication of microcapsules using two reactive polyelectrolytes (C70 and A70) to create a covalently cross-linked interpenetrating polymer network.
  • Assessment of capsule stability via mechanical stress testing (EDTA treatment) and in vivo implantation in mice for 4 weeks.
  • Evaluation of molecular weight cut-off and cell encapsulation efficacy (luciferase secretion).

Main Results:

  • The novel microcapsules demonstrated significantly enhanced mechanical and chemical resistance compared to APA capsules.
  • Confocal imaging confirmed the stability of the covalently cross-linked network after 4 weeks in vivo.
  • Encapsulated cells maintained functionality (luciferase secretion), and the modified capsules showed similar in vivo biocompatibility to APA capsules, with serum-free media preventing immune reactions.

Conclusions:

  • Covalently cross-linked microcapsules offer superior stability for gene therapy applications.
  • These enhanced microcapsules maintain essential permeability and biocompatibility for use as immunoisolation devices.
  • The developed microcapsules represent a promising advancement for cellular gene therapy delivery systems.