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Updated: Jun 27, 2026
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In situ Compressive Loading and Correlative Noninvasive Imaging of the Bone-periodontal Ligament-tooth Fibrous Joint
Published on: March 7, 2014
Morphological and molecular changes in denture-supporting tissues under persistent mechanical stress in rats
M Tsuruoka1, K Ishizaki, K Sakurai
1Department of Removable Prosthodontics and Gerodontology, Oral Health Science Center, Tokyo Dental College, Mihama-ku, Chiba, Japan.
Journal of Oral Rehabilitation
|December 19, 2008
Summary
Mechanical compression of palatal plates causes ischemia. Cells in underlying tissues, including the periosteum, produce heat shock protein 70 (HSP70) and vascular endothelial growth factor (VEGF) to maintain homeostasis.
Area of Science:
- Oral biology
- Biomaterials science
- Tissue engineering
Background:
- Mechanical forces significantly impact oral mucosa.
- Understanding cellular responses to denture base compression is crucial for tissue health.
Purpose of the Study:
- To investigate the effects of mechanical compression on rat palatal mucosa.
- To analyze the expression of heat shock protein 70 (HSP70), vascular endothelial growth factor (VEGF), and proliferation cell nuclear antigen (PCNA) under compression.
Main Methods:
- An experimental palatal base applied mechanical stress to rat palatal mucosa.
- Blood flow was measured, and tissue analysis included immunohistochemistry and mRNA expression.
- Morphometric analysis assessed proliferation cell nuclear antigen (PCNA) scores and protein expression.
Main Results:
- Mechanical compression induced ischemia in the palatal mucosa.
- PCNA scores were higher in bone-side tissues compared to epithelial-side tissues under stress.
- HSP70 and VEGF expression increased, particularly in the periosteum, peaking at 12 hours.
Conclusions:
- Mechanical compression of palatal plates leads to ischemia.
- Cells within denture-supporting tissues, especially the periosteum, upregulate HSP70 and VEGF to adapt to mechanical stress and maintain homeostasis.
