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Protein modification responds to exercise intensity and antioxidant supplementation
Manfred Lamprecht1, Karl Oettl, Guenther Schwaberger
1Institute of Physiological Chemistry, Medical University of Graz, Harrachgasse 21/II, Medical University of Graz, Graz, Austria. manfred.lamprecht@meduni-graz.at
Medicine and Science in Sports and Exercise
|December 19, 2008
Summary
Antioxidant supplementation reduced exercise-induced protein oxidation during intense exercise. Juice powder concentrate (JPC) intake lowered plasma protein carbonyls (CP) and prevented exercise-induced CP increases, suggesting a protective effect.
Area of Science:
- Exercise Physiology
- Nutritional Biochemistry
- Oxidative Stress Research
Background:
- Intense exercise can induce oxidative stress, leading to plasma protein modification.
- Antioxidant supplementation is explored as a countermeasure to exercise-induced oxidative damage.
Purpose of the Study:
- To investigate the impact of varying exercise intensities (70% vs. 80% VO2max) on plasma protein modification.
- To determine the efficacy of antioxidant supplementation (juice powder concentrate - JPC) in mitigating exercise-induced protein damage.
Main Methods:
- Trained men exercised at 70% or 80% VO2max and received either JPC or placebo for 28 weeks.
- Blood samples were collected pre- and post-exercise at multiple time points.
- Plasma protein carbonyls (CP) and the redox state of human serum albumin (HSA) were analyzed.
Main Results:
- Higher exercise intensity (80% VO2max) led to increased plasma protein carbonyls (CP).
- JPC supplementation significantly reduced baseline CP levels and blunted exercise-induced CP increases.
- Intense exercise temporarily shifted human serum albumin (HSA) to a more oxidized state.
Conclusions:
- Exercise intensity of 80% VO2max significantly increases plasma protein carbonyls.
- JPC supplementation effectively lowers baseline CP and prevents exercise-induced CP elevation.
- Intense exercise causes a reversible shift in HSA redox state, but JPC does not influence this.
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