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Updated: Jun 27, 2026
Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
Published on: December 31, 2017
DNA Checkerboard method for bacterial detection of microbiota from teeth and tongue biofilms. A preliminary study
C Do Nascimento1, S Sato, J P Mardegan Issa
1Department of Dental Materials and Prosthodontics, University of São Paulo, São Paulo, Brazil.
Aim:
The aim of this study was to determine the prevalence of 13 bacterial species in the dorsum tongue biofilm and compare its microbiota to that of subgingival samples in healthy adult subjects using DNA Checkerboard hybridization.
Methods:
A total of 40 samples, from subgingival and tongue biofilm, were enrolled into the study. All the samples were harvested and analyzed by DNA Checkerboard method for their content of 13 species of bacteria. Bacterial scores from each subject and location were averaged and the results of both sites were compared using the Wilcoxon signed ranks test.
Results:
All the microorganisms evaluated were detected in both tongue and subgingival samples, without significant differences in their prevalence.
Conclusions:
The dorsum of the tongue was colonized by various microorganisms with similar prevalence as found in the subgingival biofilm of healthy individuals, suggesting that the tongue could be a potential microbial reservoir and may play a role in the recolonization of teeth surfaces.
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