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Related Experiment Video

Updated: Jun 27, 2026

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Published on: October 9, 2016

Expression analysis and functional activity of interleukin-7 splice variants.

N K Vudattu¹, I Magalhaes, H Hoehn

¹Microbiology, Tumor and Cell Biology Center and SMI, Nobels Väg 18, Karolinska Institutet, Stockholm, Sweden.

Genes and Immunity

|December 19, 2008

Summary

Alternative splicing of human interleukin-7 (IL-7) generates functional variants. Aberrant IL-7 isoform expression in tumors suggests potential for diagnostics and therapeutics.

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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Area of Science:

Molecular Biology
Immunology
Genetics

Background:

Alternative splicing generates diverse protein isoforms from a single gene, impacting protein function.
Interleukin-7 (IL-7) is a crucial cytokine for T-cell development and survival.

Purpose of the Study:

To investigate alternative splicing of human IL-7.
To characterize the functional consequences of IL-7 splice variants.
To examine IL-7 splicing in various human tissues and disease states.

Main Methods:

Analysis of IL-7 splicing in human tissues and cell lines.
Expression and purification of recombinant IL-7 splice variants.
Assessment of IL-7 isoform function in T-cell signaling and development.

Main Results:

Extensive IL-7 splicing was observed across diverse human tissues, including granuloma lesions and tumors.
A specific IL-7 isoform lacking exon 5 induced STAT-5 phosphorylation, promoted thymocyte maturation, and enhanced T-cell survival.
Aberrant IL-7 isoform expression patterns were detected in human tumor lesions compared to non-transformed tissue.

Conclusions:

Alternative splicing of IL-7 produces functionally distinct isoforms.
Aberrant IL-7 splicing in tumors highlights its potential role in cancer.
Alternatively spliced IL-7 variants are promising candidates for diagnostic and therapeutic applications.