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Updated: Jun 27, 2026

In Vivo Assessment of Alveolar Macrophage Efferocytosis Following Ozone Exposure
Published on: October 22, 2019
In vitro reduction of mutans streptococci by means of ozone gas application
Ana Castillo1, Pablo Galindo-Moreno, Gustavo Avila
1Department of Microbiology, School of Medicine, University of Granada, Granada, Spain.
Objective:
To evaluate, in vitro, the antimicrobial effect of ozone gas on strains of reference mutans streptococci and strains isolated from children at a high risk of developing caries.
Method And Materials:
A series of dilutions was obtained from a total of 41 strains, and the effect of ozone was determined for applications of 10, 20, and 40 seconds, as well as a control, in terms of the reduction of viable bacteria.
Results:
Ozone application for 10 and 20 seconds produced a significant reduction in the number of bacteria, inversely proportional to the bacterial concentration. When exposure lasted 40 seconds, no viable bacteria were obtained.
Conclusions:
Both time of application and initial bacterial concentration bear an influence on the antimicrobial effect of ozone on mutans streptococci.
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