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UV–Vis Spectrometers

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material, molecules absorb light depending on the energy required for electronic transitions. As a result...

IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations

IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations

Identical bonds within a polyatomic group can stretch symmetrically (in-phase) or asymmetrically (out-of-phase). Similar to hydrogen bonding, these vibrations also influence the shape of the IR peak. Generally, asymmetric stretching frequencies are higher than symmetric stretching frequencies. For example, primary amines exhibit two distinct IR peaks between 3300–3500 cm−1 corresponding to the symmetric and asymmetric N-H stretching, while secondary amines exhibit a single stretching vibration...

UV–Vis Spectrum

UV–Vis Spectrum

When light passes through a substance, a portion of the light is absorbed while the remaining light is reflected or transmitted. If the molecule absorbs light between the wavelengths of 180–400 nm range, the UV spectrum is obtained, and if it absorbs light in the 400–780 nm wavelength range, the visible spectrum is obtained.
The UV–Vis spectrum of a molecule is the plot of its absorbance versus wavelength. The plot is drawn by taking molar absorptivity (ε) or log ε on the y-axis (ordinate)...

UV–Vis Spectroscopy: Woodward–Fieser Rules

UV–Vis Spectroscopy: Woodward–Fieser Rules

UV–Visible absorption spectra of conjugated dienes arise from the lowest energy π&VeryThinSpace;→&VeryThinSpace;π* transitions. The light-absorbing part of the molecule is called the chromophore, and the substituents directly attached to the chromophore are called auxochromes. A strong correlation exists between the absorption maxima, λmax, and the structure of a conjugated π system. The Woodward–Fieser rules predict the value of λmax for a given structure by adding the contributions...

Spectrophotometry: Introduction

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...

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Related Experiment Video

Updated: Jun 27, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

[White-light source spectral domain OCT based on partial spectrum analysis].

Ling Lin¹, Tae-Sok Zhang, Yu Zheng

¹State Key Laboratory of Precision Measurement Technology and Instruments, Tianjin University, Tianjin 300072, China.

Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu

|December 20, 2008

Summary

This summary is machine-generated.

A novel white-light source spectral domain optical coherence tomography (SDOCT) system improves longitudinal resolution and signal-to-noise ratio (SNR). This white-light source SDOCT (WSSDOCT) offers enhanced imaging capabilities for various applications.

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Area of Science:

Optical Engineering
Biomedical Imaging
Spectroscopy

Context:

Spectral domain optical coherence tomography (SDOCT) is a crucial imaging technique.
Enhancing longitudinal resolution and signal-to-noise ratio (SNR) are key challenges in SDOCT.
Existing SDOCT systems face limitations in achieving optimal resolution and SNR simultaneously.

Purpose:

To develop and introduce a white-light source SDOCT (WSSDOCT) system.
To enhance the longitudinal resolution and SNR of SDOCT.
To analyze the relationship between spectral characteristics and imaging performance.

Summary:

A novel WSSDOCT system was developed by analyzing the interplay between CCD pixel count, white-light spectrum, and longitudinal resolution.
Partial spectrum analysis was employed to optimize system parameters.
The system utilizes a specific white light source and detection bandwidth to improve SNR while maintaining high longitudinal resolution and detection depth.

Impact:

The developed WSSDOCT system demonstrates improved imaging performance.
Experimental results validate the system's capabilities in measuring coherence length and resolution.
The study confirms the feasibility and potential of WSSDOCT for advanced optical imaging.